Difference between revisions of "Part:BBa K3596055"

 
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<partinfo>BBa_K3596055 short</partinfo>
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BIOT is a basic design for the expression of <i>Geobacter sulfurreducens</i>' pilin-based electrically conductive protein nanowires (e-PNs)<sup>[1]</sup>.  This part codes for the monomer of <i>Geobacter sulfurreducens</i>' pilin-based electrically conductive protein nanowires(e-PNs) along with the <i>E.coli</i> PpdD signal sequence to facilitate the construction of e-PNs at N terminal.</p>
  
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===Characterization===
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<p>The e-PN can be successfully expressed by express this part under the control of pTac with typeIV pilin assembly system, and the result was verified by Western Blot(figure 1).
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<img src="https://2020.igem.org/wiki/images/4/4e/T--GreatBay_SZ--western.png" alt="" width="400">
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<h6 style="text-align:center">Figure 1: Western Blot of the cell lysis of BIOT and BIOT-His(express e-PN and e-PN-His respectively); Antibody: Anti-His tag; e-PN-His: 7.7 kDa.</h6>  </div>
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<p>We made a protein film following the instructions from the paper we researched.<sup>[1]</sup> And we eventually succeeded in producing stable voltage at about 0.35V with a current of 500nA or so.(figure 3).
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<img src="https://2020.igem.org/wiki/images/8/8f/T--GreatBay_SZ--VA.png" alt="" width="700">
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<h6 style="text-align:center">Figure 3: The voltage and current of e-PN</h6> 
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<p><b>References</b></p>
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<p>[1] Liu X, Gao H, Ward J E, <i>et al</i>. Power generation from ambient humidity using protein nanowires[J]. <i>Nature</i>, 2020, 578(7796): 550-554.</p>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3596055 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3596055 parameters</partinfo>
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Revision as of 04:50, 25 October 2020

PpdD Signal+Pilin gene

BIOT is a basic design for the expression of Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires (e-PNs)[1]. This part codes for the monomer of Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires(e-PNs) along with the E.coli PpdD signal sequence to facilitate the construction of e-PNs at N terminal.

Characterization

The e-PN can be successfully expressed by express this part under the control of pTac with typeIV pilin assembly system, and the result was verified by Western Blot(figure 1).

Figure 1: Western Blot of the cell lysis of BIOT and BIOT-His(express e-PN and e-PN-His respectively); Antibody: Anti-His tag; e-PN-His: 7.7 kDa.

We made a protein film following the instructions from the paper we researched.[1] And we eventually succeeded in producing stable voltage at about 0.35V with a current of 500nA or so.(figure 3).

Figure 3: The voltage and current of e-PN

References

[1] Liu X, Gao H, Ward J E, et al. Power generation from ambient humidity using protein nanowires[J]. Nature, 2020, 578(7796): 550-554.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]