Difference between revisions of "Part:BBa K3457009"

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[[File:T--QHFZ-China--BBa K3007000 6.png|400px|thumb|left|Figure 1. The Schematic cartoon of the DNA construct of BBa_K3457009]]
 
[[File:T--QHFZ-China--BBa K3007000 6.png|400px|thumb|left|Figure 1. The Schematic cartoon of the DNA construct of BBa_K3457009]]
  
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<p style="clear:left;">This part is the CDS of CAHS 89226. We obtained the sequence from the research paper in 2017 <sup>[1]</sup>. Then with the help of a company, we optimized the sequence, so that the part is suitable to work in <i>E. coli</i> and there is no biobrick restriction enzyme cutting site in it. Moreover, we added an 6x His tag at the N-terminal of CAHS 89226, so it can be easily detected by Western Blot and be purified by Ni-chelating affinity chromatography (Fig.1). This year, the part is used in the composite part [https://parts.igem.org/Part:K3457032 K3457032]</p>
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<p style="clear:left;">This part is the CDS of CAHS 89226. We obtained the sequence from the research paper in 2017 <sup>[1]</sup>. Then with the help of a company, we optimized the sequence, so that the part is suitable to work in <i>E. coli</i> and there is no biobrick restriction enzyme cutting site in it. Moreover, we added an 6x His tag at the N-terminal of CAHS 89226, so it can be easily detected by Western Blot and be purified by Ni-chelating affinity chromatography (Fig.1). This year, the part is used in the composite part [https://parts.igem.org/Part:BBa_K3457032 BBa_K3457032]</p>
  
  

Revision as of 03:45, 25 October 2020


CAHS 89226 with a 6X His tag

This biological is the CDS sequence of Cytosolic-abundant heat soluble protein 89226, also called CAHS 89226. It is a kind of Tardigrade intrinsically Disordered Protein (TDP). It is a heat soluble protein found from Tardigrade Hypsibius dujardini in 2017 [1]. Tardigrade, also called water bear, is a kind of tenacious organism. It can survive extreme environment, such as desiccation, freeze and vacuum. The super capacity of Tardigrade partially owes to TDPs. CAHS 89226 has been proven to protect the activity of purified proteins [1]. Here we found that expressing this protein can help bacteria survive the freeze-drying process and then the resultant dry bacteria powder can be stored for a long time at room temperature.

Contribution

Group: QHFZ-China iGEM 2020

Author: Yixian Yang

Design

Figure 1. The Schematic cartoon of the DNA construct of BBa_K3457009

This part is the CDS of CAHS 89226. We obtained the sequence from the research paper in 2017 [1]. Then with the help of a company, we optimized the sequence, so that the part is suitable to work in E. coli and there is no biobrick restriction enzyme cutting site in it. Moreover, we added an 6x His tag at the N-terminal of CAHS 89226, so it can be easily detected by Western Blot and be purified by Ni-chelating affinity chromatography (Fig.1). This year, the part is used in the composite part BBa_K3457032



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]