Difference between revisions of "Part:BBa K3606006"

Revision as of 03:44, 25 October 2020

PtetO2 promoter

This part is a fusion promoter of PL and tetO, regulated by tetR and anhydrotetracycline

Design:

This part functions to lower the leakage and raise the level of expression of the downstream gene. As a well-characterized promoter, ptetR has been studied thoroughly with its expression level and leakage. While here we take a unique idea to created a new promoter fusion: we fuse the -35 upstream, -35--10 parts of the stronger pl promoters in lambda phages with the tetO operon regulated by tetracycline. As a promoter fusion of pl and tetO, not only is it regulated by tetR and anhydrotetracycline, but it also inherited the expression level of pl.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 <h2>Design:</h2>
-This part functions to lower the leakage and raise the level of expression of the downstream gene. As a well-characterized promoter, ptetR has been studied thoroughly with its expression level and leakage. While here we take a unique idea to created a new promoter fusion: we fuse the -35 upstream, -35--10 parts of the stronger pl promoters in lambda phages with the tetO operon regulated by tetracycline. As a promoter fusion of pl and tetO, not only is it regulated by tetR and anhydrotetracycline, but it also inherited the expression level of pl。
+This part functions to lower the leakage and raise the level of expression of the downstream gene. As a well-characterized promoter, ptetR has been studied thoroughly with its expression level and leakage. While here we take a unique idea to created a new promoter fusion: we fuse the -35 upstream, -35--10 parts of the stronger pl promoters in lambda phages with the tetO operon regulated by tetracycline. As a promoter fusion of pl and tetO, not only is it regulated by tetR and anhydrotetracycline, but it also inherited the expression level of pl.
 <!-- Add more about the biology of this part here