Difference between revisions of "Part:BBa K3408002"
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We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability.
 
We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability.
  
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In our project we would use the trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could commit suicide.  
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In our project we would use the trigger RNA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacteria could commit suicide.  
  
  

Revision as of 03:41, 25 October 2020

Trigger RNA(of toehold)

It is the part of toehold switch which functions as the key to relieve switch RNA and activate downstream circuit.

We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability.

In our project we would use the trigger RNA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacteria could commit suicide.


T--NAU-CHINA--basic-parts2.png Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., Biotechnology Progress. 2010)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]