Difference between revisions of "Part:BBa K3332078"
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It can express toxin protein according to the concentration of formaldehyde.It ensures that our engineering bacteria die when the concentration of glyphosate is low to a certain level. | It can express toxin protein according to the concentration of formaldehyde.It ensures that our engineering bacteria die when the concentration of glyphosate is low to a certain level. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Formaldehyde promoter provides an inducible scheme for downstream genes and proteins. The inverter plays the role of gene inversion which reverses expression pattern when existing. MazF was designed after formaldehyde promoter and inverter, made sense of our kill switch system in degradation: formaldehyde promoter can be activated after formaldehyde induction and MazF expression is blocked and reversed by inverter. In contrast, without formaldehyde, MazF can express under the pl promoter inside inverter and cause cell death by protein toxicity. | ||
| + | [[File:T-XMU-BBa K3332078.circuit.png|none|500px|caption]] | ||
| + | Fig1. Circuit | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 03:11, 25 October 2020
Formaldehyde promoter-Inverter-MazF-terminator
It can express toxin protein according to the concentration of formaldehyde.It ensures that our engineering bacteria die when the concentration of glyphosate is low to a certain level.
Usage and Biology
Formaldehyde promoter provides an inducible scheme for downstream genes and proteins. The inverter plays the role of gene inversion which reverses expression pattern when existing. MazF was designed after formaldehyde promoter and inverter, made sense of our kill switch system in degradation: formaldehyde promoter can be activated after formaldehyde induction and MazF expression is blocked and reversed by inverter. In contrast, without formaldehyde, MazF can express under the pl promoter inside inverter and cause cell death by protein toxicity.
Fig1. Circuit Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]