Difference between revisions of "Part:BBa K3402057"
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This device is composed of 50bp-up<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402037# BBa_K3402037]), <i>hph</i>([https://parts.igem.org/Part:BBa_K3402012# BBa_K3402012]), 50bp-do<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402038# BBa_K3402038]), P<i>tef1</i>([https://parts.igem.org/Part:BBa_K3402007# BBa_K3402007]), sg<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402039# BBa_K3402039]), sg<i>GFP</i>([https://parts.igem.org/Part:BBa_K3402024# BBa_K3402024]), T<i>syn7</i>([https://parts.igem.org/Part:BBa_K3402001# BBa_K3402001]), up<i>GFP</i>([https://parts.igem.org/Part:BBa_K3402025# BBa_K3402025]), do<i>GFP</i>([https://parts.igem.org/Part:BBa_K3402026# BBa_K3402026]).
 
This device is composed of 50bp-up<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402037# BBa_K3402037]), <i>hph</i>([https://parts.igem.org/Part:BBa_K3402012# BBa_K3402012]), 50bp-do<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402038# BBa_K3402038]), P<i>tef1</i>([https://parts.igem.org/Part:BBa_K3402007# BBa_K3402007]), sg<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402039# BBa_K3402039]), sg<i>GFP</i>([https://parts.igem.org/Part:BBa_K3402024# BBa_K3402024]), T<i>syn7</i>([https://parts.igem.org/Part:BBa_K3402001# BBa_K3402001]), up<i>GFP</i>([https://parts.igem.org/Part:BBa_K3402025# BBa_K3402025]), do<i>GFP</i>([https://parts.igem.org/Part:BBa_K3402026# BBa_K3402026]).
  
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[[Image:.png|700px|center|]]
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[[Image:Double-gene editing cassette.png|600px]]
  
 
===Usage and Biology===
 
===Usage and Biology===
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We realize the knock-out of <i>PXA1</i> and <i>yeGFP</i> genes. After observing the strains that growed on the plate coated with hygromycin can also lose the function of expressing green fluorescence, we got the conclusion that the success of double gene editing was proved.
 
We realize the knock-out of <i>PXA1</i> and <i>yeGFP</i> genes. After observing the strains that growed on the plate coated with hygromycin can also lose the function of expressing green fluorescence, we got the conclusion that the success of double gene editing was proved.
  
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[[Image:.png|700px|thumb|center|Left: ]]
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[[Image:Double-gene editing cassette-plate.jpeg|400px]]
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<br>
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'''Fig. a)'''Strains can grow on the plate coated with hygromycin. '''b)'''Most of strains also lose the function of expressing green fluorescence.
  
 
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Revision as of 02:58, 25 October 2020


Double-gene editing cassette

This device is composed of 50bp-upPXA1(BBa_K3402037), hph(BBa_K3402012), 50bp-doPXA1(BBa_K3402038), Ptef1(BBa_K3402007), sgPXA1(BBa_K3402039), sgGFP(BBa_K3402024), Tsyn7(BBa_K3402001), upGFP(BBa_K3402025), doGFP(BBa_K3402026).

Double-gene editing cassette.png

Usage and Biology

We realize the knock-out of PXA1 and yeGFP genes. After observing the strains that growed on the plate coated with hygromycin can also lose the function of expressing green fluorescence, we got the conclusion that the success of double gene editing was proved.

Double-gene editing cassette-plate.jpeg
Fig. a)Strains can grow on the plate coated with hygromycin. b)Most of strains also lose the function of expressing green fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2807