Difference between revisions of "Part:BBa K3573001:Experience"
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After expression, we used Ni-NTA column for protein purification using the His6-tag located at the N-terminus of CPP-scFv(RAS). We have confirmed the identity of the protein using Western blot analysis using anti-His6 antibody. | After expression, we used Ni-NTA column for protein purification using the His6-tag located at the N-terminus of CPP-scFv(RAS). We have confirmed the identity of the protein using Western blot analysis using anti-His6 antibody. | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 02:53, 25 October 2020
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3573001
Our team has cloned scFv(RAS) into an expression vector and tested the protein expression in 4 different conditions (2 temperatures X 2 IPTG concentrations)
After expression, we used Ni-NTA column for protein purification using the His6-tag located at the N-terminus of CPP-scFv(RAS). We have confirmed the identity of the protein using Western blot analysis using anti-His6 antibody.
User Reviews
UNIQdb724d306e170bbb-partinfo-00000000-QINU UNIQdb724d306e170bbb-partinfo-00000001-QINU