Difference between revisions of "Part:BBa K117000:Design"
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===Design Notes=== | ===Design Notes=== | ||
Lysis gene was commercially synthesized by [http://www.sciencewerke.com Science Wereke] | Lysis gene was commercially synthesized by [http://www.sciencewerke.com Science Wereke] | ||
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+ | Extraction of Lysis gene/PCR Oligo fragments DNA sequence | ||
Oligo fragments with the DNA sequence of 5'-GCAGAATTCGCGGCCGCTTCTAGAG-3' & 5'-ACTAGTAGCGGCCGCTGCAGGCT-3' were used to PCR lysis gene from the blue script cloning vector. | Oligo fragments with the DNA sequence of 5'-GCAGAATTCGCGGCCGCTTCTAGAG-3' & 5'-ACTAGTAGCGGCCGCTGCAGGCT-3' were used to PCR lysis gene from the blue script cloning vector. | ||
Revision as of 16:24, 29 October 2008
Lysis gene (promotes lysis in colicin-producing bacteria strain)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Lysis gene was commercially synthesized by [http://www.sciencewerke.com Science Wereke]
Extraction of Lysis gene/PCR Oligo fragments DNA sequence Oligo fragments with the DNA sequence of 5'-GCAGAATTCGCGGCCGCTTCTAGAG-3' & 5'-ACTAGTAGCGGCCGCTGCAGGCT-3' were used to PCR lysis gene from the blue script cloning vector.
Source of Orgin sequence
to be updated.
Source
to be updated