Difference between revisions of "Part:BBa K3440000"

Revision as of 00:21, 25 October 2020

LuxI-Myc

Pconst(BBa_J23100)-RBS(BBa_B0034)-LuxI(BBa_C0061)-Myc(BBa_K823036)-DT(BBa_B0015) Will generate LuxI constitutively.

Usage and Biology

Production of LuxI under constitutive promoter. LuxI can produce 3OC6-HSL.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

Colony PCR gel for BBa_K3440000

Western blot with A3 as BBa_K3440000

Transformation plate for BBa_K3440000(A)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 698
Illegal BglII site found at 736
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 13: / Line 13: @@
 Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
 [[File:T--Stockholm--BBa_K3440000.png|thumb|center|1000px|Colony PCR gel for BBa_K3440000]]
 [[File:T--Stockholm--BBa_K3440000_WB.png|thumb|center|500px|Western blot with A3 as BBa_K3440000]]
+[[File:T--Stockholm--platea.jpg|thumb|center|500px|Transformation plate for BBa_K3440000(A)]]
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 <span class='h3bb'>Sequence and Features</span>