Difference between revisions of "Part:BBa K3440014"

(→‎Characterization)
(→‎Characterization)
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[[File:BBa_K3440000_WB.png|thumb|center|500px|Western blot with Q3 as BBa_K3440014]]
 
[[File:BBa_K3440000_WB.png|thumb|center|500px|Western blot with Q3 as BBa_K3440014]]
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[[File:T--Stockholm--greenfluo.png|thumb|center|500px|Fluorescent measurements with Q as BBa_K3440014]]
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[[File:T--Stockholm--greenfluo.png|thumb|center|500px|Fluorescent measurements with Q3 as BBa_K3440014]]
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 00:00, 25 October 2020


GFP under Plux in presence of LuxR and AHL

Pconst(BBa_J23100)-RBS(BBa_B0034)-LuxR(BBa_C0062)-Myc(BBa_K823036)-DT(BBa_B0015)-Plux(BBa_B0015)-RBS(BBa_B0034)-GFP(BBa_E0040)

Usage and Biology

This part produces GFP in the presence of 3OC6-HSL. 3OC6-HSL can from a complex with LuxR, which is expressed constitutively. the complex can activate Plux promoter, under which GFP is placed.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

Western blot with Q3 as BBa_K3440014
Fluorescent measurements with Q3 as BBa_K3440014

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 874
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1023
    Illegal BsaI.rc site found at 1750