Difference between revisions of "Part:BBa K3440010"
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===Characterization=== | ===Characterization=== | ||
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. | Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. | ||
| − | + | [[File:gelML.png|thumb|center|500px|Colony PCR gel for BBa_K3440010(M) and BBa_K3440009(L)]] | |
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Revision as of 23:55, 24 October 2020
luxI-Myc under bphR1 promoter
Pbphr1(BBa_K1155001)-RBS(BBa_B0034)-LuxI(BBa_C0061)-Myc(BBa_K823036)
Usage and Biology
Expresses LuxI under bphR1 promoter. bphR1 promoter can be activated by bphR2 or the complex bphR2:PCB. LuxI produces 3OC6-HSL, a quorum sensing molecule.
Characterization
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 963
Illegal BglII site found at 1001
Illegal BamHI site found at 156
Illegal BamHI site found at 239
Illegal XhoI site found at 46 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 139