Difference between revisions of "Part:BBa K3440007"

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Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
 
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
  
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[[File:gelGJ.png|thumb|center|500px|Colony PCR gel for BBa_K3440007(G) and BBa_K3440008(J)]]
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[[File:BBa_K3440000_WB.png|thumb|center|500px|Western blot with G7 as BBa_K3440007]]
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Revision as of 23:21, 24 October 2020


AiiA-Myc under constitutive promoter

Pconst(BBa_J23100)-RBS(BBa_B0034)-AiiA(BBa_C0060)-Myc(BBa_K823036)

Usage and Biology

Expresses AiiA under constitutive promoter. AiiA degrades lactones.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 907
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 160
  • 1000
    COMPATIBLE WITH RFC[1000]