Difference between revisions of "Part:BBa K3440005"

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[[File:plateE.png|thumb|center|50px|Transformation plate for BBa_K3440005(E)]]
 
[[File:plateE.png|thumb|center|50px|Transformation plate for BBa_K3440005(E)]]
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[[File:gelEF.png|thumb|center|500px|Transformation plate for BBa_K3440005(E)and BBa_K3440007(F)]][[File:leakinessfluo.png|thumb|center|1000px|Fluorescence measurements for BBa_K34440005 (E)]]
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[[File:gelEF.png|thumb|center|500px|Colony PCR gel for BBa_K3440005(E)and BBa_K3440007(F)]][[File:leakinessfluo.png|thumb|center|1000px|Fluorescence measurements for BBa_K34440005 (E)]]
  
 
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Revision as of 23:11, 24 October 2020


Lux I with myc tag under Promoter prmA activated in the presence of PFOS

Pprma(BBa_K2911000)-RBS(BBa_B0034)-LuxI(BBa_C0061)-Myc(BBa_K823036)

Usage and Biology

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

Transformation plate for BBa_K3440005(E)
Colony PCR gel for BBa_K3440005(E)and BBa_K3440007(F)
Fluorescence measurements for BBa_K34440005 (E)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 837
    Illegal BglII site found at 875
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]