Difference between revisions of "Part:BBa K3466007:Design"
(→Source) |
|||
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | + | cgccatgctgactgattgatcgtagtcgatgaattcgcggccgctgTAATACGACTCACTATAGgaaagaggggacaaactagaatggagaacaatctggagaacctgaccattggcgtgtttgccaaagccgccggtgtgaacgtggaaaccatccgcttctatcagcgcaaaggtctgctgcgcgaaccggataaaccgtatggcagcattcgccgctatggtgaagccgatgtggtgcgcgtgaaattcgtgaaaagcgcccagcgtctgggctttagcctggacgagattgccgactttctgcgcattgacgaaggctgtgtgggtcatgacgccctggccgaacataaactgaaggatgtgcgcgagaaaatggccgacctggcccgcatggaaaccgtgctgagcgagctgatgtgtaatctgccgccgggcaatgtgagctgtccgctgattgaaagcttacaaggtgaagcaggtctggcccgtagcgcaatgccgtaattgactctgtagttgctacagggtgtgcaattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaatcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagtagcggccgctgcagtgcgttagcgtacattacagtcagctagct | |
| − | + | ||
===References=== | ===References=== | ||
Latest revision as of 22:57, 24 October 2020
T7 -> RBS -> MerR -> PcadA -> RBS -> mRFP -> TT
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 277
Illegal AgeI site found at 379
Illegal AgeI site found at 1152
Illegal AgeI site found at 1264 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used a T7 promoter to test for better compatibility with the Sigma 70 Master Mix from Arbor Biosciences.
Source
cgccatgctgactgattgatcgtagtcgatgaattcgcggccgctgTAATACGACTCACTATAGgaaagaggggacaaactagaatggagaacaatctggagaacctgaccattggcgtgtttgccaaagccgccggtgtgaacgtggaaaccatccgcttctatcagcgcaaaggtctgctgcgcgaaccggataaaccgtatggcagcattcgccgctatggtgaagccgatgtggtgcgcgtgaaattcgtgaaaagcgcccagcgtctgggctttagcctggacgagattgccgactttctgcgcattgacgaaggctgtgtgggtcatgacgccctggccgaacataaactgaaggatgtgcgcgagaaaatggccgacctggcccgcatggaaaccgtgctgagcgagctgatgtgtaatctgccgccgggcaatgtgagctgtccgctgattgaaagcttacaaggtgaagcaggtctggcccgtagcgcaatgccgtaattgactctgtagttgctacagggtgtgcaattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaatcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagtagcggccgctgcagtgcgttagcgtacattacagtcagctagct