Difference between revisions of "Part:BBa K3440001"
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===Characterization=== | ===Characterization=== | ||
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. | Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. | ||
| − | [[File:BBa_K34440001 and BBa_K3440002_WB.png|thumb|center| | + | [[File:BBa_K34440001 and BBa_K3440002_WB.png|thumb|center|500px|Western Blot for BBa_K34440001 and BBa_K3440002]] |
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Revision as of 22:23, 24 October 2020
mCherry under bphR1 promoter
PbphR1(BBa_K1155001)-RBS(BBa_B0034)-mCherry(BBa_J06504)-DT(BBa_B0015)
Usage and Biology
Produces mCherry under bphR1 promoter, which can be activated by bphR2:Polychlorinated biphenyl.
Characterization
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 156
Illegal BamHI site found at 239
Illegal XhoI site found at 46 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 139