Difference between revisions of "Part:BBa K3466002:Design"

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===Source===
 
===Source===
  
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Constitutive promoter - BBa_J23100, RBS+MerR - BBa_K1724002, PcadA promoter - BBa_K1724000, RBS - BBa_J34801, mRFP - BBa_I13521, TT - BBa_B0015
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ttgacggctagctcagtcctaggtacagtgctagcgaaagaggggacaaactagaatggagaacaatctggagaacctgaccattggcgtgtttgccaaagccgccggtgtgaacgtggaaaccatccgcttctatcagcgcaaaggtctgctgcgcgaaccggataaaccgtatggcagcattcgccgctatggtgaagccgatgtggtgcgcgtgaaattcgtgaaaagcgcccagcgtctgggctttagcctggacgagattgccgactttctgcgcattgacgaaggcaccggttgtgtgggtcatgacgccctggccgaacataaactgaaggatgtgcgcgagaaaatggccgacctggcccgcatggaaaccgtgctgagcgagctgaccggtatgtgtaatctgccgccgggcaatgtgagctgtccgctgattgaaagcttacaaggtgaagcaggtctggcccgtagcgcaatgccgtaattgactctgtagttgctacagggtgtgcaataaagaggagaaatccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
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===References===
 
===References===

Latest revision as of 22:04, 24 October 2020


CP -> RBS -> MerR -> PcadA -> RBS -> mRFP -> TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 293
    Illegal AgeI site found at 395
    Illegal AgeI site found at 1168
    Illegal AgeI site found at 1280
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used a spacer to test whether or not it serves to enhance our RFP expression rate.


Source

Constitutive promoter - BBa_J23100, RBS+MerR - BBa_K1724002, PcadA promoter - BBa_K1724000, RBS - BBa_J34801, mRFP - BBa_I13521, TT - BBa_B0015

References