Difference between revisions of "Part:BBa K3440012"

Revision as of 15:57, 24 October 2020

pRhl promoter with GFP activated by RhlR expression and AHL

Pconst(BBa_J23100)-RBS(BBa_B0034)-RHlR(BBa_C0071)-Myc(BBa_K823036)-DT(BBa_B0015)-Prhl-RBS-GFP(BBa_K1949060)

Usage and Biology

This part expresses GFP in the presence of C4-HSL. RhlR is constitutively expressed. In the presence of C4-HSL, RhlR:C¤-HSL complex can activate Prhl, which controls production of GFP.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 880
Illegal BamHI site found at 301
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 776
Illegal BsaI.rc site found at 1754

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
+This part expresses GFP in the presence of C4-HSL. RhlR is constitutively expressed. In the presence of C4-HSL, RhlR:C¤-HSL complex can activate Prhl, which controls production of GFP.
 ===Characterization===
 Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.