Difference between revisions of "Part:BBa K3402056"
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This device is composed of 50bp-up<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402037# BBa_K3402037]), <i>hph</i>([https://parts.igem.org/Part:BBa_K3402012# BBa_K3402012]), 50bp-do<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402038# BBa_K3402038]), P<i>tef1</i>([https://parts.igem.org/Part:BBa_K3402007# BBa_K3402007]), sg<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402039# BBa_K3402039]), T<i>syn7</i>([https://parts.igem.org/Part:BBa_K3402001# BBa_K3402001]). | This device is composed of 50bp-up<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402037# BBa_K3402037]), <i>hph</i>([https://parts.igem.org/Part:BBa_K3402012# BBa_K3402012]), 50bp-do<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402038# BBa_K3402038]), P<i>tef1</i>([https://parts.igem.org/Part:BBa_K3402007# BBa_K3402007]), sg<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402039# BBa_K3402039]), T<i>syn7</i>([https://parts.igem.org/Part:BBa_K3402001# BBa_K3402001]). | ||
| − | [[Image:.png| | + | [[Image:Single-gene editing cassette sequence.png|600px]] |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 15:39, 24 October 2020
Single-gene editing cassette
This device is composed of 50bp-upPXA1(BBa_K3402037), hph(BBa_K3402012), 50bp-doPXA1(BBa_K3402038), Ptef1(BBa_K3402007), sgPXA1(BBa_K3402039), Tsyn7(BBa_K3402001).
Usage and Biology
We achieve the knockout of PXA1 gene and insert the hygromycin resistant gene as a selection marker. Two parts of PXA1 are homologous arms at the ends.
If modified strains can grow on the plate coated with hygromycin, it is successful to knock PXA1 with Cas9 system. By comparison, the editing efficiency of the CRISPR/Cas9 system can be clearly observed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2171
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]