Difference between revisions of "Part:BBa K3440015"
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===Usage===
 
===Usage===
−
This is an intermediate site used in the construction of BBa_K3440020 and BBa_K3440021
+
This is an intermediate part used in the construction of BBa_K3440020 and BBa_K3440021
  
 
===Characterization===
 
===Characterization===

Revision as of 15:35, 24 October 2020


RhlI-AiiA

RhlI(BBa_C0070)-RBS(BBa_B0034)-AiiA(BBa_C0060)

Usage

This is an intermediate part used in the construction of BBa_K3440020 and BBa_K3440021

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 661
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 792
  • 1000
    COMPATIBLE WITH RFC[1000]