Difference between revisions of "Part:BBa K3402046"
 
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We used green fluorescence protein <i>yeGFP</i>([https://parts.igem.org/Part:BBa_K3402000# BBa_K3402000]) to characterize the strength of this promoter in <i>S. bombicola</i>.
 
We used green fluorescence protein <i>yeGFP</i>([https://parts.igem.org/Part:BBa_K3402000# BBa_K3402000]) to characterize the strength of this promoter in <i>S. bombicola</i>.
  
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[[Image:Expression of Pcyp52m1 by yeGFP.png|700px|thumb|center|Expression of P<i>cyp52m1</i> by <i>yeGFP</i>]]
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[[Image:Fluorescence intensity and transcription level of different promoters.png|700px|thumb|center|'''Left:''' Fluorescence intensity, '''Right:''' Transcription level]]
  
 
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Latest revision as of 15:30, 24 October 2020


Pcyp52m1

Pcyp52m1 is the promoter of Cytochrome P450 monooxygenase from S. bombicola.

Usage and Biology

We used green fluorescence protein yeGFP(BBa_K3402000) to characterize the strength of this promoter in S. bombicola.

Left: Fluorescence intensity, Right: Transcription level

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 638
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 257
    Illegal AgeI site found at 1097
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 286