Difference between revisions of "Part:BBa K3402044"
 
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We used green fluorescence protein <i>yeGFP</i>([https://parts.igem.org/Part:BBa_K3402000# BBa_K3402000]) to characterize the strength of this promoter in <i>S. bombicola</i>.
 
We used green fluorescence protein <i>yeGFP</i>([https://parts.igem.org/Part:BBa_K3402000# BBa_K3402000]) to characterize the strength of this promoter in <i>S. bombicola</i>.
  
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[[Image:Expression of Pmob by yeGFP.png|700px|thumb|center|Expression of P<i>mob</i> by <i>yeGFP</i>]]
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[[Image:Fluorescence intensity and transcription level of different promoters.png|700px|thumb|center|'''Left:''' Fluorescence intensity, '''Right:''' Transcription level]]
  
 
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Latest revision as of 15:27, 24 October 2020


Pmob

Pmob is the promoter of monooxygenase B from S. bombicola.

Usage and Biology

We used green fluorescence protein yeGFP(BBa_K3402000) to characterize the strength of this promoter in S. bombicola.

Left: Fluorescence intensity, Right: Transcription level

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 26
    Illegal BglII site found at 684
    Illegal XhoI site found at 341
    Illegal XhoI site found at 612
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 103
    Illegal SapI site found at 630
    Illegal SapI site found at 815