Difference between revisions of "Part:BBa K3402007"

 
Line 9: Line 9:
 
We used green fluorescence protein <i>yeGFP</i>([https://parts.igem.org/Part:BBa_K3402000# BBa_K3402000]) to characterize the strength of this promoter in <i>S. bombicola</i>.
 
We used green fluorescence protein <i>yeGFP</i>([https://parts.igem.org/Part:BBa_K3402000# BBa_K3402000]) to characterize the strength of this promoter in <i>S. bombicola</i>.
  
[[Image:Expression of Ptef1 by yeGFP.png|700px|thumb|center|Expression of P<i>tef1</i> by <i>yeGFP</i>]]
+
[[Image:Expression of Ptef1 by yeGFP.png|700px|thumb|center|'''Left:''' Expression of P<i>tef1</i> by <i>yeGFP</i>, '''Middle:''' Fluorescence intensity, '''Right:''' Transcription level]]
  
 
<!-- -->
 
<!-- -->

Latest revision as of 15:21, 24 October 2020


Ptef1

Ptef1 is the promoter of the transcription elongation factor 1 from Starmerella bombicola.

Usage and Biology

We used green fluorescence protein yeGFP(BBa_K3402000) to characterize the strength of this promoter in S. bombicola.

Left: Expression of Ptef1 by yeGFP, Middle: Fluorescence intensity, Right: Transcription level

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1051
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]