Difference between revisions of "Part:BBa K3440018"
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===Usage and Biology===
 
===Usage and Biology===
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===Characterization===
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Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
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This is the final construct to detect PCB and consequently produce a quorum sensing molecule. bphR2 is produced constitutively and can bind to a PCB. The complex PCB:bphR2 can activate bphR1 promoter, under which LuxI is placed. LuxI produces the 3OC6-HSL quorum sensing molecule.
  
  

Revision as of 15:17, 24 October 2020


PCB detection

Pconst(BBa_J23100)-RBS(BBa_B0034)-bphR2(BBa_K1413021)-Myc(BBa_K823036)-DT(BBa_B0015)-Pbphr1(BBa_K1155001)-RBS(BBa_B0034)-LuxI(BBa_C0061)

Usage and Biology

This is the final construct to detect PCB and consequently produce a quorum sensing molecule. bphR2 is produced constitutively and can bind to a PCB. The complex PCB:bphR2 can activate bphR1 promoter, under which LuxI is placed. LuxI produces the 3OC6-HSL quorum sensing molecule.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 996
    Illegal BglII site found at 2108
    Illegal BamHI site found at 1301
    Illegal BamHI site found at 1384
    Illegal XhoI site found at 1191
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 320
    Illegal SapI.rc site found at 1284