Difference between revisions of "Part:BBa K3440019"
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===Usage and Biology===
 
===Usage and Biology===
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===Characterization===
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This part can be used to produce mtrb (gene responsible for electricity production in Shewanella oneidensis) in the presence of 3OC6-HSL.  
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Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
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Revision as of 15:13, 24 October 2020


MtrB under Lux promoter

Pconst(BBa_J23100)-RBS(BBa_B0034)-LuxR(BBa_C0062)-DT(BBa_B0015)-Plux(BBa_R0062)-RBS(BBa_B0034)-mtrb(BBa_K3146001)-DT(BBa_B0015)

Usage and Biology

This part can be used to produce mtrb (gene responsible for electricity production in Shewanella oneidensis) in the presence of 3OC6-HSL.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985