According to research, HicA is a toxin promoting mRNA degradation, leading to the hibernation of bacteria, while HicB is an antitoxin that can neutralize the effect of HicA. Hence, through regulating the expression of hicA and hicB genes, we can control the growth of bacteria, hibernating the bacteria before the contact lens is used.
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<br>Overview
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Since contact lens are sealed during the process of production, it is impossible to regulate the growth switch through chemical induction; hence, we turned to external stimuli such as light, temperature, etc. At first, we used EL222 to regulate the system (a blue light-sensitive protein), pBlind (a promoter activated by EL222), FLP, and FRT to change the orientation of our promoter, which is activated by blue light. In other words, we have to illuminate the contact lens with blue light for 30 minutes before use.
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First, the production stage is when we are culturing our bacteria. Without any arabinose addition during culture, the bacteria will be able to grow normally. After the production process, we will put the bacteria and arabinose into the contact lens together, so the pBAD promoter will transcribe hicA, causing the bacteria to hibernate. When we need to resuscitate the bacteria, EL222 will be activated by blue light to induce pBlind promoter, and then FLP will be transcribed. FLP will act on the FRT sites to change the orientation of the constitutive promoter, as a result, the bacteria will start to transcribe hicB to neutralize HicA and thus continue to grow.
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Revision as of 15:13, 24 October 2020
Regulating the growth rate of bacteria.
Overview
Sequence and Features
Assembly Compatibility:
10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1512 Illegal SpeI site found at 1830
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1512 Illegal NheI site found at 7 Illegal NheI site found at 30 Illegal NheI site found at 2789 Illegal SpeI site found at 1830
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1512 Illegal BamHI site found at 2729
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1512 Illegal SpeI site found at 1830
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1512 Illegal SpeI site found at 1830 Illegal NgoMIV site found at 142 Illegal AgeI site found at 367
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2225 Illegal BsaI.rc site found at 2265
