Difference between revisions of "Part:BBa K2804002"

Latest revision as of 14:57, 24 October 2020

Bone morphogenetic protein 2 (BMP2)

The human bone morphogenetic protein 2 is a bone-growth regulatory factor well known to be a potent osteoinductive protein.

Characterization of BMP2's effects in cartilage regeneration (by KEYSTONE_A 2020)

BMP2 and VEGF inhibition promote expression of cartilaginous pathways on sorted skeletal stem-cell (SSC) in vitro (Figure 3). Skeletal stem-cells (SSCs) isolated from the articular surface, periosteum and bone marrow all produced higher amounts of cartilage with addition of BMP2 + sVEGFR1 when compared with addition of PBS only (Figure 4). For in vivo experiments, cotransplantation with BMP2 + sVEGFR1 can also promote cartilage formation (Figure 5).

Reference

Murphy, M. P., Koepke, L. S., Lopez, M. T., Tong, X., Ambrosi, T. H., Gulati, G. S., ... & Steininger, H. (2020). Articular cartilage regeneration by activated skeletal stem cells. Nature Medicine, 1-10.

Figure 3. SSC gene expression levels after in vitro treatment of PBS, BMP2, or sVEGFR1.

Figure 4. Alcian blue staining (for acidic glycans in cartilage) of SSC following chondrogenic differentiation with treatment of PBS or BMP2 + sVEGFR1

Figure 5. Cotransplantation with BMP2 + sVEGFR1 led to increased cartilage formation in vivo

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 =='''Characterization of BMP2's effects in cartilage regeneration (by KEYSTONE_A 2020)'''==
-BMP2 and VEGF inhibition promote expression of cartilaginous pathways on sorted skeletal stem-cell (SSC) in vitro '''(Figure 3)'''.
+BMP2 and VEGF inhibition promote expression of cartilaginous pathways on sorted skeletal stem-cell (SSC) in vitro '''(Figure 3)'''. Skeletal stem-cells (SSCs) isolated from the articular surface, periosteum and bone marrow all produced higher amounts of cartilage with addition of BMP2 + sVEGFR1 when compared with addition of PBS only '''(Figure 4)'''. For in vivo experiments, cotransplantation with BMP2 + sVEGFR1 can also promote cartilage formation '''(Figure 5)'''.
-Skeletal stem-cells (SSCs) isolated from the articular surface, periosteum and bone marrow all produced higher amounts of cartilage with addition of BMP2 + sVEGFR1 when compared with addition of PBS only '''(Figure 4)'''.
 '''Reference'''
@@ Line 18: / Line 18: @@
 [[File:KEYSTONE A BMP2 VEGFR effect_2.jpg|600px|thumb|left|'''Figure 4. Alcian blue staining (for acidic glycans in cartilage) of SSC following chondrogenic differentiation with treatment of PBS or BMP2 + sVEGFR1''']]
+[[File:KEYSTONE A BMP2 VEGFR effect_in vivo.jpg|600px|thumb|left|'''Figure 5. Cotransplantation with BMP2 + sVEGFR1 led to increased cartilage formation in vivo''']]