Difference between revisions of "Part:BBa K3440017"
Line 13: Line 13:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3440017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3440017 SequenceAndFeatures</partinfo>
−
jhjhj
+
 
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 14:39, 24 October 2020


bphR2 under bphR1 promoter

Pconst(BBa_J23100)-RBS(BBa_B0034)-bphR2 mutated(BBa_K1413021)-Myc(BBa_K823036)-DT(BBa_B0015)-Pbphr1(BBa_K1155001)-RBS(BBa_B0034)-mCherry(BBa_J06504)

Usage and Biology

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 996
    Illegal BamHI site found at 1301
    Illegal BamHI site found at 1384
    Illegal XhoI site found at 1191
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 320
    Illegal SapI.rc site found at 1284