Difference between revisions of "Part:BBa K3440010"

 
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Pbphr1(BBa_K1155001)-RBS(BBa_B0034)-LuxI(BBa_C0061)-Myc(BBa_K823036)
 
Pbphr1(BBa_K1155001)-RBS(BBa_B0034)-LuxI(BBa_C0061)-Myc(BBa_K823036)
  
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===Usage and Biology===
 
===Usage and Biology===
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===Characterization===
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Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
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Revision as of 14:36, 24 October 2020


luxI-Myc under bphR1 promoter

Pbphr1(BBa_K1155001)-RBS(BBa_B0034)-LuxI(BBa_C0061)-Myc(BBa_K823036)

Usage and Biology

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 963
    Illegal BglII site found at 1001
    Illegal BamHI site found at 156
    Illegal BamHI site found at 239
    Illegal XhoI site found at 46
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 139