Difference between revisions of "Part:BBa K3505011"

Revision as of 13:19, 24 October 2020

pDGB1 omega 2 (Ω2) for Bacterial GoldenBraid

Level Ω vector for Golden Braid Cloning. Having cargo LacZa for blue white screening between BsaI and BsmBI sites.Resistance in Spectinomycin and ori pBBR1 for multiple protein expression capacities.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 3277
Illegal XbaI site found at 3304
Illegal PstI site found at 3316
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 3277
Illegal PstI site found at 3316
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 3277
Illegal BamHI site found at 3298
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 3277
Illegal XbaI site found at 3304
Illegal PstI site found at 3316
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 3277
Illegal XbaI site found at 3304
Illegal PstI site found at 3316
Illegal NgoMIV site found at 948
Illegal NgoMIV site found at 1350
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2918
Illegal BsaI.rc site found at 6

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3505011 short</partinfo>
-LONG
+Level Ω vector for Golden Braid Cloning. Having cargo LacZa for blue white screening between BsaI and BsmBI sites.Resistance in Spectinomycin and ori pBBR1 for multiple protein expression capacities.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+===Sequence and Features===
-<span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3505011 SequenceAndFeatures</partinfo>
-<!-- Uncomment this to enable Functional Parameter display
-===Functional Parameters===
-<partinfo>BBa_K3505011 parameters</partinfo>
-<!-- -->