Difference between revisions of "Part:BBa K133001"

 
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<partinfo>BBa_K133001 short</partinfo>
 
<partinfo>BBa_K133001 short</partinfo>
  
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chimeric flagellin with H.pylori multiepitope
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Chimeric flagellin with added multiepitope at the C-terminal. Chimeric fusion protein retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of ''E. coli'' with that of ''H. pylori'' enables activation of innate immunity - a characteristic that flagellin of ''H. pylori'' alone does not posess - while presenting the antigen of ''H. pylori''  for antibody production. We also designed a highly immunogenic fusion protein - multiepitope - which combines most immunogenic portions of three essensial ''H. pylori''
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:38, 29 October 2008

CF-multiHP-RGD-Histop

Chimeric flagellin with added multiepitope at the C-terminal. Chimeric fusion protein retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of E. coli with that of H. pylori enables activation of innate immunity - a characteristic that flagellin of H. pylori alone does not posess - while presenting the antigen of H. pylori for antibody production. We also designed a highly immunogenic fusion protein - multiepitope - which combines most immunogenic portions of three essensial H. pylori

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1207
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 764
    Illegal BamHI site found at 1275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2091