Difference between revisions of "Part:BBa K3468032:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
NONE
+
By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. V149I was designed by searching for suitable sites around K148 for mutation.
  
  

Latest revision as of 11:22, 24 October 2020


PETase V149I


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. V149I was designed by searching for suitable sites around K148 for mutation.


Source

Ideonalla sakaiensis

References