Difference between revisions of "Part:BBa K3468079:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
NONE
+
Reading the literature found that the i+1 position of β-turn has an obvious residue preference for pro. The introduction of proline mutations can theoretically improve thermal stability.
  
  

Latest revision as of 11:12, 24 October 2020


PETase T113P


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 240
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 240
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 240
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Reading the literature found that the i+1 position of β-turn has an obvious residue preference for pro. The introduction of proline mutations can theoretically improve thermal stability.


Source

Ideonalla sakaiensis

References