Difference between revisions of "Part:BBa K3519009"

Revision as of 11:02, 24 October 2020

medium promoter-traT

This part has the traT gene (BBa_K3519003) downstream to the medium strength Anderson promoter (BBa_J23118), the strong RBS (BBa_B0034) and is followed by the double terminator (BBa_B0015). Constitutive overexpression of the traT gene helps reduce the formation of stable mating pairs. The data provided below are from previous overexpression studies in the literature.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 703
Illegal BsaI.rc site found at 185

Revision as of 11:01, 24 October 2020 (view source) Omkarmohapatra123 (Talk \| contribs) ← Older edit		Revision as of 11:02, 24 October 2020 (view source) Omkarmohapatra123 (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K3519009 short</partinfo>		<partinfo>BBa_K3519009 short</partinfo>

−	This part has the traT gene (BBa_K3519003) downstream to the medium strength Anderson promoter (BBa_J23118), the strong RBS (BBa_B0034) and is followed by the double terminator (BBa_B0015). Constitutive overexpression of the traT gene helps reduce the formation of stable mating pairs.	+	This part has the traT gene (BBa_K3519003) downstream to the medium strength Anderson promoter (BBa_J23118), the strong RBS (BBa_B0034) and is followed by the double terminator (BBa_B0015). Constitutive overexpression of the traT gene helps reduce the formation of stable mating pairs. The data provided below are from previous overexpression studies in the literature.