Difference between revisions of "Part:BBa K3505040"
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[[File:T--Thessaly--a1r.png|600px|thumb|none|<i><b>Fig.1:</b> a1R plasmid Features and Restiction Sites.</i>]]
 
[[File:T--Thessaly--a1r.png|600px|thumb|none|<i><b>Fig.1:</b> a1R plasmid Features and Restiction Sites.</i>]]
 
[[File:T--Thessaly--a2.png|600px|thumb|none|<i><b>Fig.1:</b> a2 plasmid Features and Restiction Sites.</i>]]
 
[[File:T--Thessaly--a2.png|600px|thumb|none|<i><b>Fig.1:</b> a2 plasmid Features and Restiction Sites.</i>]]
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===Usage and Biology===
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===Experimental Use and Experience===
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The following parts were cloned in these 2 vectors
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<bbpart>BBa_K3505025</bbpart>
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<bbpart>BBa_K3505026</bbpart>
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<bbpart>BBa_K3505027</bbpart>
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<bbpart>BBa_K3505028</bbpart>
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<bbpart>BBa_K3505029</bbpart>
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<bbpart>BBa_K3505030</bbpart>
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<bbpart>BBa_K3505031</bbpart>
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<bbpart>BBa_K3505032</bbpart>
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<bbpart>BBa_K3505033</bbpart>
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<bbpart>BBa_K3505034</bbpart>
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<bbpart>BBa_K3505035</bbpart>
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<bbpart>BBa_K3505038</bbpart>
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<bbpart>BBa_K3505039</bbpart>
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=pDGB1 a1R verification with no insert=
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[[File:T--Thessaly--alpha2.png|500px|thumb|none|<i><b>Fig.3:</b>  pDGB1 a1R Digested with BamHI. Expected bands 2847, 385, 239 and pDGB1 a2 Digested with HindIII. Expected bands 2847, 416, 206</i>]]
 
===Sequence and Features===
 
===Sequence and Features===
 
<partinfo>BBa_K3505040 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3505040 SequenceAndFeatures</partinfo>
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===References===
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*[1]Alejandro Sarrion-Perdigones, Marta Vazquez-Vilar, Jorge Palací, Bas Castelijns, Javier Forment, Peio Ziarsolo, José Blanca, Antonio Granell, Diego Orzaez (2013). “GoldenBraid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic Biology.” <em>Plant Physiology</em> , 162 (3) 1618-1631; DOI: 10.1104/pp.113.217661
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*[2]Esteban Martínez-García, Angel Goñi-Moreno, Bryan Bartley, James McLaughlin, Lucas Sánchez-Sampedro, Héctor Pascual del Pozo, Clara Prieto Hernández, Ada Serena Marletta, Davide De Lucrezia, Guzmán Sánchez-Fernández, Sofía Fraile, Víctor de Lorenzo, SEVA 3.0: an update of the Standard European Vector Architecture for enabling portability of genetic constructs among diverse bacterial hosts,<em> Nucleic Acids Research</em>, Volume 48, Issue D1, 08 January 2020, Pages D1164–D1170, https://doi.org/10.1093/nar/gkz1024

Revision as of 10:38, 24 October 2020


pDGB1 alpha BACKBONE for Bacterial GoldenBraid Cloning

This backbone combined with the proper insert BBa_K3505041 results in the comlpete Vectors BBa_K3505008BBa_K3505009

Fig.1: a1R plasmid Features and Restiction Sites.
Fig.1: a2 plasmid Features and Restiction Sites.

Usage and Biology

Experimental Use and Experience

The following parts were cloned in these 2 vectors BBa_K3505025 BBa_K3505026 BBa_K3505027 BBa_K3505028 BBa_K3505029 BBa_K3505030 BBa_K3505031 BBa_K3505032 BBa_K3505033 BBa_K3505034 BBa_K3505035 BBa_K3505038 BBa_K3505039

pDGB1 a1R verification with no insert

Fig.3: pDGB1 a1R Digested with BamHI. Expected bands 2847, 385, 239 and pDGB1 a2 Digested with HindIII. Expected bands 2847, 416, 206

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1274
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  • [1]Alejandro Sarrion-Perdigones, Marta Vazquez-Vilar, Jorge Palací, Bas Castelijns, Javier Forment, Peio Ziarsolo, José Blanca, Antonio Granell, Diego Orzaez (2013). “GoldenBraid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic Biology.” Plant Physiology , 162 (3) 1618-1631; DOI: 10.1104/pp.113.217661
  • [2]Esteban Martínez-García, Angel Goñi-Moreno, Bryan Bartley, James McLaughlin, Lucas Sánchez-Sampedro, Héctor Pascual del Pozo, Clara Prieto Hernández, Ada Serena Marletta, Davide De Lucrezia, Guzmán Sánchez-Fernández, Sofía Fraile, Víctor de Lorenzo, SEVA 3.0: an update of the Standard European Vector Architecture for enabling portability of genetic constructs among diverse bacterial hosts, Nucleic Acids Research, Volume 48, Issue D1, 08 January 2020, Pages D1164–D1170, https://doi.org/10.1093/nar/gkz1024