Difference between revisions of "Part:BBa K3505041"
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<b>This LacZa moudule is the cargo of the Bacterial GoldenBraid Plasmid Collection. </b> | <b>This LacZa moudule is the cargo of the Bacterial GoldenBraid Plasmid Collection. </b> | ||
=This LacZa module is flanked by the proper BsaI and BsmBI sites giving ID to the backbones= | =This LacZa module is flanked by the proper BsaI and BsmBI sites giving ID to the backbones= | ||
| − | *pUPD2 backbone <bbpart>BBa_K3505043</bbpart>+ LacZa pUPD2 <b>=</b> <bbpart>BBa_K3505007</bbpart> | + | *pUPD2 backbone <bbpart>BBa_K3505043</bbpart>+ LacZa pUPD2 <b>=</b> <bbpart>BBa_K3505007</bbpart> |
| − | *alpha backbone <bbpart>BBa_K3505040</bbpart>+ LacZa α1R <b>=</b> <bbpart>BBa_K3505008</bbpart> | + | *alpha backbone <bbpart>BBa_K3505040</bbpart>+ LacZa α1R <b>=</b> <bbpart>BBa_K3505008</bbpart> |
| − | *alpha backbone <bbpart>BBa_K3505040</bbpart>+ LacZa α2 <b>=</b> <bbpart>BBa_K3505009</bbpart> | + | *alpha backbone <bbpart>BBa_K3505040</bbpart>+ LacZa α2 <b>=</b> <bbpart>BBa_K3505009</bbpart> |
| − | *omega backbone <bbpart>BBa_K3505042</bbpart>+ LacZa Ω1 <b>=</b> <bbpart>BBa_K3505010</bbpart> | + | *omega backbone <bbpart>BBa_K3505042</bbpart>+ LacZa Ω1 <b>=</b> <bbpart>BBa_K3505010</bbpart> |
| − | *omega backbone <bbpart>BBa_K3505042</bbpart>+ LacZa Ω2 <b>=</b> <bbpart>BBa_K3505011</bbpart> | + | *omega backbone <bbpart>BBa_K3505042</bbpart>+ LacZa Ω2 <b>=</b> <bbpart>BBa_K3505011</bbpart> |
[[File:T--Thessaly--LacZa.png|800px|thumb|none|<i><b>Fig.1:</b> The LacZa module flanking sites for every GB pLamid type</i>]] | [[File:T--Thessaly--LacZa.png|800px|thumb|none|<i><b>Fig.1:</b> The LacZa module flanking sites for every GB pLamid type</i>]] | ||
[[File:T--Thessaly--GBcloninggrammar.jpeg|900px|thumb|none|<i><b>Fig.1:</b> The GoldenBraid Garmmar</i>]] | [[File:T--Thessaly--GBcloninggrammar.jpeg|900px|thumb|none|<i><b>Fig.1:</b> The GoldenBraid Garmmar</i>]] | ||
Revision as of 10:20, 24 October 2020
LacZa for Bacterial GoldenBraid plasmids
This LacZa moudule is the cargo of the Bacterial GoldenBraid Plasmid Collection.
This LacZa module is flanked by the proper BsaI and BsmBI sites giving ID to the backbones
- pUPD2 backbone BBa_K3505043+ LacZa pUPD2 = BBa_K3505007
- alpha backbone BBa_K3505040+ LacZa α1R = BBa_K3505008
- alpha backbone BBa_K3505040+ LacZa α2 = BBa_K3505009
- omega backbone BBa_K3505042+ LacZa Ω1 = BBa_K3505010
- omega backbone BBa_K3505042+ LacZa Ω2 = BBa_K3505011
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 366
Illegal PstI site found at 405 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 366
Illegal BamHI site found at 387 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7
Illegal BsaI.rc site found at 616