Difference between revisions of "Part:BBa K3505041"
Line 3: Line 3:
 
<partinfo>BBa_K3505041 short</partinfo>
 
<partinfo>BBa_K3505041 short</partinfo>
  
−
<b>This LacZa moudule is the cargo of the Bacterial Golden Braid Plasmid Collection </b>
+
<b>This LacZa moudule is the cargo of the Bacterial GoldenBraid Plasmid Collection. </b>
 +
=This LacZa module is flanked by the proper BsaI and BsmBI sites giving ID to the backbones=
 
*pUPD2 backbone <bbpart>BBa_K3505043</bbpart>+ LacZa pUPD2 <b>=</b> <bbpart>BBa_K3505007</bbpart>
 
*pUPD2 backbone <bbpart>BBa_K3505043</bbpart>+ LacZa pUPD2 <b>=</b> <bbpart>BBa_K3505007</bbpart>
 
*alpha backbone <bbpart>BBa_K3505040</bbpart>+ LacZa α1R <b>=</b> <bbpart>BBa_K3505008</bbpart>
 
*alpha backbone <bbpart>BBa_K3505040</bbpart>+ LacZa α1R <b>=</b> <bbpart>BBa_K3505008</bbpart>

Revision as of 10:11, 24 October 2020


LacZa for Bacterial GoldenBraid plasmids

This LacZa moudule is the cargo of the Bacterial GoldenBraid Plasmid Collection.

This LacZa module is flanked by the proper BsaI and BsmBI sites giving ID to the backbones

Fig.1: The LacZa module flanking sites for every GB pLamid type
Fig.1: The GoldenBraid Garmmar

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 366
    Illegal PstI site found at 405
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 366
    Illegal BamHI site found at 387
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 616