Difference between revisions of "Part:BBa K3505041"
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<b>This LacZa moudule is the cargo of the Bacterial Golden Braid Plasmid Collection </b> | <b>This LacZa moudule is the cargo of the Bacterial Golden Braid Plasmid Collection </b> | ||
| − | *pUPD2 backbone <bbpart>BBa_K3505043</bbpart>+ LacZa pUPD2<b>=<b> <bbpart>BBa_K3505007</bbpart> | + | *pUPD2 backbone <bbpart>BBa_K3505043</bbpart>+ LacZa pUPD2 <b>=</b> <bbpart>BBa_K3505007</bbpart> |
| − | *alpha backbone <bbpart>BBa_K3505040</bbpart>+ LacZa α1R<b>=</b> <bbpart>BBa_K3505008</bbpart> | + | *alpha backbone <bbpart>BBa_K3505040</bbpart>+ LacZa α1R <b>=</b> <bbpart>BBa_K3505008</bbpart> |
| − | *alpha backbone <bbpart>BBa_K3505040</bbpart>+ LacZa α2<b>=</b> <bbpart>BBa_K3505009</bbpart> | + | *alpha backbone <bbpart>BBa_K3505040</bbpart>+ LacZa α2 <b>=</b> <bbpart>BBa_K3505009</bbpart> |
| − | *omega backbone <bbpart>BBa_K3505042</bbpart>+ LacZa Ω1<b>=</b> <bbpart>BBa_K3505010</bbpart> | + | *omega backbone <bbpart>BBa_K3505042</bbpart>+ LacZa Ω1 <b>=</b> <bbpart>BBa_K3505010</bbpart> |
| − | *omega backbone <bbpart>BBa_K3505042</bbpart>+ LacZa Ω2<b>=</b> <bbpart>BBa_K3505011</bbpart> | + | *omega backbone <bbpart>BBa_K3505042</bbpart>+ LacZa Ω2 <b>=</b> <bbpart>BBa_K3505011</bbpart> |
[[File:T--Thessaly--LacZa.png|800px|thumb|none|<i><b>Fig.1:</b> The LacZa module flanking sites for every GB pLamid type</i>]] | [[File:T--Thessaly--LacZa.png|800px|thumb|none|<i><b>Fig.1:</b> The LacZa module flanking sites for every GB pLamid type</i>]] | ||
[[File:T--Thessaly--GBcloninggrammar.jpeg|900px|thumb|none|<i><b>Fig.1:</b> The GoldenBraid Garmmar</i>]] | [[File:T--Thessaly--GBcloninggrammar.jpeg|900px|thumb|none|<i><b>Fig.1:</b> The GoldenBraid Garmmar</i>]] | ||
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===Sequence and Features=== | ===Sequence and Features=== | ||
<partinfo>BBa_K3505041 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3505041 SequenceAndFeatures</partinfo> | ||
Revision as of 10:04, 24 October 2020
LacZa for Bacterial GoldenBraid plasmids
This LacZa moudule is the cargo of the Bacterial Golden Braid Plasmid Collection
- pUPD2 backbone BBa_K3505043+ LacZa pUPD2 = BBa_K3505007
- alpha backbone BBa_K3505040+ LacZa α1R = BBa_K3505008
- alpha backbone BBa_K3505040+ LacZa α2 = BBa_K3505009
- omega backbone BBa_K3505042+ LacZa Ω1 = BBa_K3505010
- omega backbone BBa_K3505042+ LacZa Ω2 = BBa_K3505011
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 366
Illegal PstI site found at 405 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 366
Illegal BamHI site found at 387 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7
Illegal BsaI.rc site found at 616