Difference between revisions of "Part:BBa K3505041"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3505041 short</partinfo>
 
<partinfo>BBa_K3505041 short</partinfo>
 +
 
<b>This LacZa moudule is the cargo of the Bacterial Golden Braid Plasmid Collection </b>
 
<b>This LacZa moudule is the cargo of the Bacterial Golden Braid Plasmid Collection </b>
 
[[File:T--Thessaly--LacZa.png|500px|thumb|none|<i><b>Fig.1:</b> The LacZa module flanking sites for every GB pLamid type</i>]]
 
[[File:T--Thessaly--LacZa.png|500px|thumb|none|<i><b>Fig.1:</b> The LacZa module flanking sites for every GB pLamid type</i>]]
[[File:T--Thessaly--Goldenbraidgrammar.jpeg|500px|thumb|none|<i><b>Fig.1:</b> The GoldenBraid Garmmar</i>]]
+
[[File:T--Thessaly--GBcloninggrammar.jpeg|500px|thumb|none|<i><b>Fig.1:</b> The GoldenBraid Garmmar</i>]]
 
===Usage and Biology===
 
===Usage and Biology===
  
 
===Sequence and Features===
 
===Sequence and Features===
 
<partinfo>BBa_K3505041 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3505041 SequenceAndFeatures</partinfo>

Revision as of 09:51, 24 October 2020


LacZa for Bacterial GoldenBraid plasmids

This LacZa moudule is the cargo of the Bacterial Golden Braid Plasmid Collection

Fig.1: The LacZa module flanking sites for every GB pLamid type
Fig.1: The GoldenBraid Garmmar

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 366
    Illegal PstI site found at 405
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 366
    Illegal BamHI site found at 387
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 616