Difference between revisions of "Part:BBa K3633011"
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| + | ==References== | ||
| + | 1. M. Guerrero‐Rubio, R. López‐Llorca, P. Henarejos‐Escudero, F. García‐Carmona and F. Gandía‐Herrero, "Scaled‐up biotechnological production of individual betalains in a microbial system", Microbial Biotechnology, vol. 12, no. 5, pp. 993-1002, 2019. Available: 10.1111/1751-7915.13452. | ||
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| + | 2. "Betalaine", De.wikipedia.org, 2020. [Online]. Available: https://de.wikipedia.org/wiki/Betalaine. [Accessed: Jun-2020]. | ||
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| + | 3. G. Polturak and A. Aharoni, "“La Vie en Rose”: Biosynthesis, Sources, and Applications of Betalain Pigments", Molecular Plant, vol. 11, no. 1, pp. 7-22, 2018. Available: 10.1016/j.molp.2017.10.008. | ||
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| + | 4. P. Grewal, C. Modavi, Z. Russ, N. Harris and J. Dueber, "Bioproduction of a betalain color palette in Saccharomyces cerevisiae", Metabolic Engineering, vol. 45, pp. 180-188, 2018. Available: 10.1016/j.ymben.2017.12.008. | ||
Revision as of 09:35, 24 October 2020
A composite part to express 4,5-DODA, responsible for production of betalains
Description
Betalains are water-soluble nitrogen-containing pigments that are subdivided in red-violet betacyanins and yellow-orange betaxanthins. Due to glycosylation and acylation betalains exhibit a huge structural diversity. Betanin (betanidin-5-O--glucoside) is the most common betacyanin in the plant kingdom.
The biosynthesis of betalains in plants excludes that of anthocyanins. During the biosynthesis of betalains in the cytoplasm three enzymes are involved: Tyrosinase, 4,5-DOPA-extradioldioxygenase, and betanidin-glucosyltransferase. The amino acid L-tyrosine, which is enzymatically formed over the shikimate pathway from arogenic acid, is the precursor for the biosynthesis of L-DOPA. Tyrosine is hydroxylated by means of the enzyme tyrosinase to DOPA (I) that is formed to betalamic acid or to cyclo-DOPA. The biosynthesis of betalamic acid, which is the basic structure of betalains as follow: 4,5-DOPA-extradiol dioxygenase opens the cyclic ring of L-DOPA between carbons 4 and 5, thus producing 4,5-seco-DOPA (II). This intermediate product occurs naturally. Due to spontaneous intramolecular condensation between the amine group and the aldehyde group of 4,5-seco-DOPA betalamic acid is formed.
In order to make the production of the Betacyanin, Shanghai_SFLS_SPBS built the biobrick with 4,5-DODA and J23102 promoter and added the substrate L-Dopa. The 4,5-seco-DOPA will spontaneously convert into Betalamic acid with the help of ascorbic acid(Vitamin C).And Dopaxanthin/Indoline-Betacyanin will be subsequently synthesized by adding the substrate of L-DOPA/Indoline. However, the production of betalains is not obvious for this biobrick.
Experiments & Results
No successful production of betalains was achieved.
Sequence & Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
1. M. Guerrero‐Rubio, R. López‐Llorca, P. Henarejos‐Escudero, F. García‐Carmona and F. Gandía‐Herrero, "Scaled‐up biotechnological production of individual betalains in a microbial system", Microbial Biotechnology, vol. 12, no. 5, pp. 993-1002, 2019. Available: 10.1111/1751-7915.13452.
2. "Betalaine", De.wikipedia.org, 2020. [Online]. Available: https://de.wikipedia.org/wiki/Betalaine. [Accessed: Jun-2020].
3. G. Polturak and A. Aharoni, "“La Vie en Rose”: Biosynthesis, Sources, and Applications of Betalain Pigments", Molecular Plant, vol. 11, no. 1, pp. 7-22, 2018. Available: 10.1016/j.molp.2017.10.008.
4. P. Grewal, C. Modavi, Z. Russ, N. Harris and J. Dueber, "Bioproduction of a betalain color palette in Saccharomyces cerevisiae", Metabolic Engineering, vol. 45, pp. 180-188, 2018. Available: 10.1016/j.ymben.2017.12.008.