Difference between revisions of "Part:BBa K3633008"

Revision as of 07:21, 24 October 2020

A composite part to express Tyr1, responsible for melanin production

Description

Fig 1. Melanin Synthesis Pathway

In this part, the gene of Tyr1 is added to promoter J23102-RBS.

Tyr gene provides instructions for making enzyme tyrosinase which is located in melanocytes in our skin. Melanocytes are responsible for producing melanin. Melanin is responsible for giving our skin and hair color. It includes eumelanin and pheomelanin. Eumelanin usually shows darker color than pheomelanin. As shown below, tyrosinase is responsible for the first step in melanin production.

Tyrosine is one of the 20 standard amino acids present in the body and used by cells to synthesize proteins. This is a non-essential amino acid, meaning that when it is not only ingested from the diet, it is also synthesized by the body. Tyrosine is found in casein, a protein in milk and other milk-based products (like cheese). Tyrosine is also present in non-dairy foods that are aged, including some types of meats and red wine.

Experiments & Results

Successful production in E.coli BL21(DE3) and vibrio natriegens

We constructed the plasmid J23102-Tyr1 and successfully transformed the plasmid into E. coli BL21(DE3) and Vibrio natriegens ATCC 14048. We added 2.5 mg L-DOPA and 0.4 mg L-Tyrosine as substrates and cultured the bacteria in shakers (220 rpm) at 25℃ or 37℃.

Fig 2. Melanin Synthesis Pathway

Sequence & Features