Difference between revisions of "Part:BBa K3594008"
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<partinfo>BBa_K3594008 short</partinfo> | <partinfo>BBa_K3594008 short</partinfo> | ||
| + | ==Usage and Biology== | ||
This composite part is used as the serotonin degradation device. It is composed of the basic parts BBa_K3594008, Monoamine Oxidase (MAOA) gene BBa_K1615022 and Aldose reductase (ALDR) gene BBa_K1602004. | This composite part is used as the serotonin degradation device. It is composed of the basic parts BBa_K3594008, Monoamine Oxidase (MAOA) gene BBa_K1615022 and Aldose reductase (ALDR) gene BBa_K1602004. | ||
| + | [[File:T--SHSBNU gene circuit of serotonin degrading enzymes.jpeg|400px|thumb|center|gene circuit of BBa_K3594008]] | ||
| + | ==Characterization== | ||
| + | [[File:T--SHSBNU China--SDS-PAGE.png|500px|thumb|center|The analysis of SDS-PAGE of MAOA&ALDR]] | ||
| + | According to the testing result of SDS-PAGE, although we have successfully constructed the plasmid, MAO is not expressed by the bacteria. Therefore, we used mathematical modelling to predict the concentration of MAO and other following effect. | ||
| + | We assume that the change of protein concentration in the external environment is related to the amount of gene expression, the rate of bacterial lysis, the rate of protein degradation, and the rate of locust intestinal peristalsis. | ||
| + | [[File:T--SHSBNU China--changes in protein concentration.jpeg|400px|thumb|center|]] | ||
| + | And got the following results: | ||
| + | [[File:T--SHSBNU_China--concentration of enzymes.jpg|400px|thumb|center|The concentration of the protein changes over time. The abscissa represents time]] | ||
| + | The ordinate represents the protein concentration. It can be seen from the image that the protein increases rapidly after the bacterial self-lysis. After reaching a certain concentration, due to the degradation rate of the protein itself and the locust intestinal peristalsis. The rate decreases slightly, and the concentration increases with the next bacterial self-lysis, which forms a stable cycle that can release enzymes efficiently. | ||
| + | |||
| + | [[File:T--SHSBNU China--serotonin goes down.jpeg|400px|thumb|center|The concentration of serotonin changes over time]] | ||
| + | The abscissa represents time, and the ordinate is the concentration of serotonin in the locust. It can be seen from the image that the concentration of serotonin decline over time. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 06:44, 24 October 2020
Serotonin Degrading Enzymes System
Usage and Biology
This composite part is used as the serotonin degradation device. It is composed of the basic parts BBa_K3594008, Monoamine Oxidase (MAOA) gene BBa_K1615022 and Aldose reductase (ALDR) gene BBa_K1602004.
Characterization
According to the testing result of SDS-PAGE, although we have successfully constructed the plasmid, MAO is not expressed by the bacteria. Therefore, we used mathematical modelling to predict the concentration of MAO and other following effect. We assume that the change of protein concentration in the external environment is related to the amount of gene expression, the rate of bacterial lysis, the rate of protein degradation, and the rate of locust intestinal peristalsis.
And got the following results:
The ordinate represents the protein concentration. It can be seen from the image that the protein increases rapidly after the bacterial self-lysis. After reaching a certain concentration, due to the degradation rate of the protein itself and the locust intestinal peristalsis. The rate decreases slightly, and the concentration increases with the next bacterial self-lysis, which forms a stable cycle that can release enzymes efficiently.
The abscissa represents time, and the ordinate is the concentration of serotonin in the locust. It can be seen from the image that the concentration of serotonin decline over time. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]