Difference between revisions of "Part:BBa K3365000"

 
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<partinfo>BBa_K3365000 short</partinfo>
 
<partinfo>BBa_K3365000 short</partinfo>
  
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&#969; is fused at the C-terminal end of dCas9. This fusion protein is the transcription activator and inhibitor.  
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RpoZ, an RNAP &#969; subunit which can activate transcription by recruiting the RNAP holoenzyme is fused to dCas9 C-terminal to activate gene transcription downstream of the protospacer.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:58, 24 October 2020


dCas9-ω

RpoZ, an RNAP ω subunit which can activate transcription by recruiting the RNAP holoenzyme is fused to dCas9 C-terminal to activate gene transcription downstream of the protospacer.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1340
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1340
    Illegal BamHI site found at 3378
    Illegal BamHI site found at 4212
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1340
  • 1000
    COMPATIBLE WITH RFC[1000]