Difference between revisions of "Part:BBa K3697000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | mCherry is a valuable tool for usage in B. subtilis due to its strong potential as a reporter protein. Colonies expression mCherry_BSU at high levels from strong constitutive promotors such as pVeg can appear red to the naked eye. mCherry_BSU can be expressed by both E. coli and B. subtilis, and is not tagged for degredation, giving a strong, enduring signal. | ||
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Revision as of 00:16, 24 October 2020
mCherry BSU
This is the coding sequence for producing a codon-optimized mCherry in B. subtilis. mCherry is a derivative of RFP. mCherry_BSU was created by codon-optimizing an E. coli mCherry for expression B. subtilis. When paired with a strong RBS and constitutive promotor (BBa_K3697010), mCherry_BSU can be quantified using a plate reader, and transformed B. subtilis colonies can be moderately red to the naked eye.
mCherry_BSU has an excitation peak at 585 nm and a peak emission at 615 nm.
Fluorescence levels in B. subtilis and E. coli can be quantified using a fluorescent plate reader. Under high enough expression from a strong constitutive promotor such as pVeg (BBa_K143012), red colonies may be visible to the naked eye. E. coli expressing mCherry_BSU are red under natural light.
This protein can be used as a reporter in B. subtilis due to it's high visibility.
This part does not have a degradation tag.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 715 - 1000COMPATIBLE WITH RFC[1000]