Difference between revisions of "Part:BBa K3389002:Design"

 
(Design Notes)
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===Design Notes===
 
===Design Notes===
There were no special design considerations. This is the complete coding sequence for the flappase protein.
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This sequence was optimized for expression in Escherichia coli.
 
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===Source===
 
===Source===

Revision as of 22:40, 23 October 2020


RAD27 Flappase coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 273
    Illegal BglII site found at 666
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 178


Design Notes

This sequence was optimized for expression in Escherichia coli.

Source

The source of this sequence is the genomic sequence from Saccharomyces cerevisiae, the RAD27 gene (GenBank accession number: EF628290.1).

References