Difference between revisions of "Part:BBa K3360002"
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| + | Beta-glucosidase is a cellulase enzyme, which can catalyze the reaction of geniposide with amino acids to produce gardenia blue pigment. In order to find an enzyme that has a good tolerance to the extreme industrial environment and can achieve rapid color development in laboratory conditions, we finally created an extremely heat-resistant β-glucosidase derived from Thermotoga marina. | ||
| + | We chose the plasmid pGEX-4T-1-H, and added a GST tag to it and optimized its gene sequence for E. coli codons. Under IPTG induction, the enzyme can be expressed efficiently. | ||
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| + | [[File: Plasmid vector diagram.png|200px|center]] | ||
| + | <center>xxxxxxxxxxxxxxxx</center> | ||
Revision as of 22:11, 23 October 2020
Thermostable beta-glucosidase
Encoding the β-glucosidase A of Thermotoga maritima, the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1577
Illegal AgeI site found at 927 - 1000COMPATIBLE WITH RFC[1000]
SZU-China 2020 TEAM
Beta-glucosidase is a cellulase enzyme, which can catalyze the reaction of geniposide with amino acids to produce gardenia blue pigment. In order to find an enzyme that has a good tolerance to the extreme industrial environment and can achieve rapid color development in laboratory conditions, we finally created an extremely heat-resistant β-glucosidase derived from Thermotoga marina.
We chose the plasmid pGEX-4T-1-H, and added a GST tag to it and optimized its gene sequence for E. coli codons. Under IPTG induction, the enzyme can be expressed efficiently.
