Difference between revisions of "Part:BBa K3338023"

 
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<partinfo>BBa_K3338023 parameters</partinfo>
 
<partinfo>BBa_K3338023 parameters</partinfo>
 
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=References=
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Goldhawk, D. E., Lemaire, C., McCreary, C. R., McGirr, R., Dhanvantari, S., Thompson, R. T., Figueredo, R., Koropatnick, J., Foster, P., & Prato, F. S. (2009). Magnetic resonance imaging of cells overexpressing MagA, an endogenous contrast agent for live cell imaging. <i>Molecular imaging</i>, 8(3), 129–139.
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Tannous B. A. (2009). Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo. <i>Nature protocols</i>, 4(4), 582–591.
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Zurkiya, O., Chan, A. W., & Hu, X. (2008). MagA is sufficient for producing magnetic nanoparticles in mammalian cells, making it an MRI reporter. <i>Magnetic resonance in medicine</i>, 59(6), 1225–1231.

Revision as of 22:10, 23 October 2020


IL-6 Pmut-MagA-P2A-hGLuc

IL-6 P-MagA-P2A-hGLuc is an inflammatory toxin sensor comprising the IL6-Promoter downstream from the MagA-P2A-hGLuc cassette. This part combines the LPS-sensitivity of the IL6-Promoter with the simultaneous expression of the reporter genes MagA for MRI-detection and Gaussia Luciferase for bioluminescence detection in blood or urine (Goldhawk et al. 2009, Zurkiya et al. 2008, Tannous 2009). The construct displays the first step in generating a functional inflammatory toxin sensor for clinical use.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 220
    Illegal BamHI site found at 395
    Illegal XhoI site found at 1581
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 340
    Illegal BsaI site found at 2803
    Illegal BsaI.rc site found at 387
    Illegal BsaI.rc site found at 696
    Illegal BsaI.rc site found at 2135
    Illegal BsaI.rc site found at 2684
    Illegal SapI site found at 1825


References

Goldhawk, D. E., Lemaire, C., McCreary, C. R., McGirr, R., Dhanvantari, S., Thompson, R. T., Figueredo, R., Koropatnick, J., Foster, P., & Prato, F. S. (2009). Magnetic resonance imaging of cells overexpressing MagA, an endogenous contrast agent for live cell imaging. Molecular imaging, 8(3), 129–139.

Tannous B. A. (2009). Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo. Nature protocols, 4(4), 582–591.

Zurkiya, O., Chan, A. W., & Hu, X. (2008). MagA is sufficient for producing magnetic nanoparticles in mammalian cells, making it an MRI reporter. Magnetic resonance in medicine, 59(6), 1225–1231.