Difference between revisions of "Part:BBa K3360002:Design"

(→‎Design Notes)
(→‎Source)
Line 11: Line 11:
 
===Source===
 
===Source===
  
−
It conmes from Thermotoga maritima.
+
Encoding a β-glucosidase derived from Thermotoga maritima. Its heat resistance has been greatly improved, and the optimum temperature is 90°C.The N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.The entire sequence has been optimized by E.coli codons
  
 
===References===
 
===References===

Revision as of 22:03, 23 October 2020


Thermostable beta-glucosidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1577
    Illegal AgeI site found at 927
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Encoding a β-glucosidase derived from Thermotoga maritima. Its heat resistance has been greatly improved, and the optimum temperature is 90°C.The N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.The entire sequence has been optimized by E.coli codons

Source

Encoding a β-glucosidase derived from Thermotoga maritima. Its heat resistance has been greatly improved, and the optimum temperature is 90°C.The N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.The entire sequence has been optimized by E.coli codons

References