Difference between revisions of "Part:BBa K3346002:Design"
 
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===Design Notes===
 
===Design Notes===
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Mutations were introduced at residues 382 and 428 to allow efficient binding to Fc receptors. We also performed codon optimization for an E. coli chassis using Reverse Translate Software available on The Sequence Manipulation Site and chose alternative codons to remove illegal restriction sites within the sequence.
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Mutations were introduced at residues 382 and 428 to allow efficient binding to Fc receptors. We also performed codon optimization for an E. coli chassis using Reverse Translate Software available on The Sequence Manipulation Suite and chose alternative codons to remove illegal restriction sites within the sequence.
  
  

Latest revision as of 21:25, 23 October 2020


Murine Constant Chain for IgG Expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutations were introduced at residues 382 and 428 to allow efficient binding to Fc receptors. We also performed codon optimization for an E. coli chassis using Reverse Translate Software available on The Sequence Manipulation Suite and chose alternative codons to remove illegal restriction sites within the sequence.


Source

The sequence was available on the UniProt database. This sequence is from the murine genome.

References