Difference between revisions of "Part:BBa K3338019"

Revision as of 21:23, 23 October 2020

IL-6 P_mut-hGLuc

This part consists of an interleukin-6 promoter that is mutagenized to make it compatible with the RFC10 assembly standard that controls the expression of the reporter hGLuc. It can be used to determine the basal promoter activity and the activity following changes in the surrounding of the cell like LPS supplementation. As a control CMV-hGLuc (BBa_K3338017) can be used.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1347
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 220
Illegal BamHI site found at 395
Illegal XhoI site found at 1581
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 340
Illegal BsaI.rc site found at 387
Illegal BsaI.rc site found at 696

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3338019 short</partinfo>
-This part consists of an interleukin-6 promoter that is mutagenized to make it compatible with the RFC10 assembly standard that controls the expression of the reporter hGLuc. It can be used to determine the basal promoter activity and the activity following changes in the surrounding of the cell like LPS supplementation. As a control CMV-hGLuc (<html><a href="https://parts.igem.org/Part:BBa_K3338017"> BBa_K3338017</a></html>) can be used.
+This part consists of an interleukin-6 promoter that is mutagenized to make it compatible with the RFC10 assembly standard that controls the expression of the reporter hGLuc. It can be used to determine the basal promoter activity and the activity following changes in the surrounding of the cell like LPS supplementation. As a control CMV-hGLuc (<html><a href="https://parts.igem.org/Part:BBa_K3338017">BBa_K3338017</a></html>) can be used.
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