Difference between revisions of "Part:BBa K3692002"
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<partinfo>BBa_K3692002 short</partinfo> | <partinfo>BBa_K3692002 short</partinfo> | ||
− | + | ==Usage and Biology== | |
''PLIN3'' is a gene that encodes perilipin - a protein which is involved both in generation of lipid droplets (LDs) and as well as covering of phospholipid monolayer of LDs. | ''PLIN3'' is a gene that encodes perilipin - a protein which is involved both in generation of lipid droplets (LDs) and as well as covering of phospholipid monolayer of LDs. | ||
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*Teixeira, P. G., David, F., Siewers, V., & Nielsen, J. (2018). Engineering lipid droplet assembly mechanisms for improved triacylglycerol accumulation in Saccharomyces cerevisiae. FEMS Yeast Research, 18(6). https://doi.org/10.1093/femsyr/foy060 | *Teixeira, P. G., David, F., Siewers, V., & Nielsen, J. (2018). Engineering lipid droplet assembly mechanisms for improved triacylglycerol accumulation in Saccharomyces cerevisiae. FEMS Yeast Research, 18(6). https://doi.org/10.1093/femsyr/foy060 | ||
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− | + | ==Sequence and Features== | |
<partinfo>BBa_K3692002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3692002 SequenceAndFeatures</partinfo> | ||
Revision as of 21:13, 23 October 2020
PLIN3
Usage and Biology
PLIN3 is a gene that encodes perilipin - a protein which is involved both in generation of lipid droplets (LDs) and as well as covering of phospholipid monolayer of LDs.
PLIN3 is a human perilipin gene. However, it has been shown that the expression of this gene can increase yields of TAG by 28% in Sacharomyces cerevisiae (Teixeira et al., 2018). A potential reason for this can be that it stabilizes TAGs by improving LD assembly and the budding process.
References
- Teixeira, P. G., David, F., Siewers, V., & Nielsen, J. (2018). Engineering lipid droplet assembly mechanisms for improved triacylglycerol accumulation in Saccharomyces cerevisiae. FEMS Yeast Research, 18(6). https://doi.org/10.1093/femsyr/foy060
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 369
Illegal PstI site found at 218 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 369
Illegal PstI site found at 218 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 369
Illegal PstI site found at 218 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 369
Illegal PstI site found at 218 - 1000COMPATIBLE WITH RFC[1000]